SSG3242 TISSUE CULTURE TECHNOLOGY (TEKNOLOGI KULTUR TISU)

Topic outline

• General
  

  General

  Lecturer : Dr Alina Wagiran Dr Siti Pauliena Mohd Bohari Dr Salehhuddin Hamdan Semester : Semester I 2010/2011
  Synopsis : The course will cover historical aspect of plant and animal tissue culture, biology of cultured cells, design and layout of the cell culture laboratory, equipments and handling of the tissue culture. Aseptic technique, general safety, culture vessels and media preparation & sterilization will be discussed. Other topics will include cultivation of plant & animal cells and tissues and some important applications.
  This work,SSG3242 Tissue Culture Technology by Siti Pauliena Binti Mohd Bohari is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License

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  Introduction to Animal Cell culture (Chapter 1)
  

  At the end of this topic students will be able to understand a basic concepts in animal cell culture technology. This includes :general terms that usually use in animal cell culture, tissue culture applications nowadays, differentiate between primary cells and establish cell lines.

  
  

  • Introduction to animal cell culture File
  • Classification of animal cells and tissues File
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  Topic 9

  Laboratory requirements and aseptic techniques (Chapter 2)
  

  At the end of this chapter students will be able to recognize all of the important laboratory equipments and its functions, learn how to have a good aseptic techniques when handling animal cells in lab and learn how to avoid or reduce contamination while culturing cells.

  
  

  • Equipment and Lab design File
  • Aseptic Technique and Lab Design File
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  Topic 10

  Defined media and supplements (Chapter 3)
  

  This chapter discuss on the basic media requirements, types of sera that can be used, supplements that are needed by different types of cells and physicochemical properties that could affect cell growth.

  
  

  • Defined media and supplements File
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  Topic 11

  Primary culture, subculture and cell line, cryopreservation (Chapter 4)

  In this chapter students will be exposed in details about primary and cell lines culture, how to do the subculture when the cells reach their confluency. At the last part, students will learn about the biological of the cultured cells that includes the extracellular matrix, cytoskeleton, cell adhesion molecules, etc.

  
  

  • Primary culture, subculture, cell lines and cryopreservation File
  • Biology of Cultured Cells File
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  Cloning and selection(Chapter 5)
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  Animal cell culture application in technology (Chapter 6)
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