Lecturer : Dr Alina Wagiran
Dr Siti Pauliena Mohd Bohari
Dr Salehhuddin Hamdan
Semester : Semester I 2010/2011
The course will cover historical aspect of plant and animal tissue culture, biology of cultured cells, design and layout of the cell culture laboratory, equipments and handling of the tissue culture. Aseptic technique, general safety, culture vessels and media preparation & sterilization will be discussed. Other topics will include cultivation of plant & animal cells and tissues and some important applications.
This work,SSG3242 Tissue Culture Technology by Siti Pauliena Binti Mohd Bohari is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License
- Topic 8
Topic 8Introduction to Animal Cell culture (Chapter 1)
At the end of this topic students will be able to understand a basic concepts in animal cell culture technology. This includes :general terms that usually use in animal cell culture, tissue culture applications nowadays, differentiate between primary cells and establish cell lines.
- Topic 9
Topic 9Laboratory requirements and aseptic techniques (Chapter 2)
At the end of this chapter students will be able to recognize all of the important laboratory equipments and its functions, learn how to have a good aseptic techniques when handling animal cells in lab and learn how to avoid or reduce contamination while culturing cells.
- Topic 10
Topic 10Defined media and supplements (Chapter 3)
This chapter discuss on the basic media requirements, types of sera that can be used, supplements that are needed by different types of cells and physicochemical properties that could affect cell growth.
- Topic 11
Primary culture, subculture and cell line, cryopreservation (Chapter 4)
In this chapter students will be exposed in details about primary and cell lines culture, how to do the subculture when the cells reach their confluency. At the last part, students will learn about the biological of the cultured cells that includes the extracellular matrix, cytoskeleton, cell adhesion molecules, etc.
- Topic 12
Topic 12Cloning and selection(Chapter 5)
- Topic 13
Topic 13Animal cell culture application in technology (Chapter 6)